voltage clamp - определение. Что такое voltage clamp
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Что (кто) такое voltage clamp - определение


Voltage clamp         
  • A personal photo of Kenneth Cole, given to Dr. J. Walter Woodbury
The voltage clamp is an experimental method used by electrophysiologists to measure the ion currents through the membranes of excitable cells, such as neurons, while holding the membrane voltage at a set level. A basic voltage clamp will iteratively measure the membrane potential, and then change the membrane potential (voltage) to a desired value by adding the necessary current.
Patch clamp         
  • Cell-attached patch configuration
  • Inside-out patch configuration
  • Loose patch clamp technique
  • Outside-out patch formation technique. In order: top-left, top-right, bottom-left, bottom-right
  • Classical patch clamp setup, with [[microscope]], antivibration table, and [[micromanipulator]]s
  • Circuit formed during whole-cell or perforated patch clamp
  • Typical equipment used during classical patch clamp recording
  • Diagram showing variations of the patch clamp technique
  • Perforated patch technique
  • Schematic depiction of a pipette puller device used to prepare micropipettes for patch clamp and other recordings
  • A patch clamp recording of current reveals transitions between two conductance states of a single ion channel: closed (at top) and open (at bottom).
  • Patch clamp of a nerve cell within a slice of brain tissue. The pipette in the photograph has been marked with a slight blue color.
  • Whole-cell patch configuration
LABORATORY TECHNIQUE IN ELECTROPHYSIOLOGY USED TO STUDY IONIC CURRENTS IN INDIVIDUAL ISOLATED LIVING CELLS, TISSUE SECTIONS, OR PATCHES OF CELL MEMBRANE
Patch clamp recording; Patch-clamp technique; Patch-clamp; Whole Cell Patch Clamp; Whole-cell patch clamping; Whole-cell patch clamp; Patch clamping; Whole-cell recording; Patch-clamp techniques
The patch clamp technique is a laboratory technique in electrophysiology used to study ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane. The technique is especially useful in the study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells, and can also be applied to the study of bacterial ion channels in specially prepared giant spheroplasts.
Cutoff voltage         
VOLTAGE AT WHICH A BATTERY IS CONSIDERED FULLY DISCHARGED, BEYOND WHICH FURTHER DISCHARGE COULD CAUSE HARM
Cut-off voltage; Low-voltage-cutoff
In electronics, the cut-off voltage is the voltage at which a battery is considered fully discharged, beyond which further discharge could cause harm. Some electronic devices, such as cell phones, will automatically shut down when the cut-off voltage has been reached.